Fluorescence Flow Injection Analysis System
 The
FIA-PMT-FL
is a highly sensitive -- to parts per trillion -- photomultiplier-based
flow through detection system ideally suited for ultra-low fluorescence,
chemiluminescence or bioluminescence measurements. The system's
heavy-duty, chemically resistant housing is built to withstand
industrial environments.
The FIA-PMT-FL has
flow-through capability, but also accepts standard
1-cm path length cuvettes for manual
measurements. The FIA-PMT-FL is $6,495.
System Configuration
The FIA-PMT is built to order with either
an internal excitation lamp or with an optical fiber interfacing to an
external lamp. Excitation lamp source options include tungsten, mercury
vapor, LEDs and laser diodes. Emission and excitation filters are
mounted in accessible slots inside the enclosure, allowing for easy
removal or exchange. The system uses an internal 25
mm
flow cell.
Excitation Source Options
|
Internal excitation source options: |
Laser diodes (532 nm, 635 nm, 650 nm) |
|
Quartz tungsten lamp (440-750 nm) |
|
UV LED lamp (365-410 nm) |
|
Various Visible LED lamps |
|
Fiber optic excitation source options: |
Deuterium lamp (200-400 nm) |
|
Laser diodes (532 nm, 635 nm, 650 nm) |
|
Quartz tungsten lamp (440-750 nm) |
System Operation
The FIA-PMT-FL
plugs into the RS-232 serial port of a personal computer and comes with
Windows-based software that allows the user to set the integration time
and voltage counts, and to obtain time histories of the measurements,
both plotted and tabulated. Also, the software can automatically create
calibration curves, and control a host of additional devices including
various syringe and peristaltic pumps, injection valves, selection
valves, and autosamplers (please inquire for details). For users who
desire to control the PMT-FL from their own software project, included
is an ActiveX control for use with Visual Basic, VC++, LabvVew, or
nearly any other ActiveX- compatible development environment.
Typical Applications
Fluorometric ammonium analysis. Use the FIA-PMT-FL
for the orthophtaldialdehyde (OPA) method for NH4 assays, to ppb
sensitivity. Results achieved with 365 nm LED excitation source with
broadband 410-490 nm emission filter.
Fluorescein-based assays. Use the FIA-PMT-FL to
measure to ppb sensitivity. Results achieved with Blue LED excitation
source and broadband 510-590 nm emission filter.
Additional Options
- Heated internal flow cell -- ambient to 60
°C, +/- 0.5 °C
accuracy
- Dual-head PMT -- measure two excitation
wavelength bands simultaneously
Specifications
|
Dimensions: |
~13 cm x 18 cm x 25.4 cm |
|
Spectral range: |
310-750 nm (filter/lamp selection
dependent) |
|
Detector: |
Photo-counting photomultiplier tube |
|
PMT dynamic range: |
2 x 10^6 |
|
Responsivity: |
5 x 10^17 cps/watt (@ 400 nm) |
|
Detection limits: |
10 parts per trillion measured with
fluorescein @ 200 msec integration time and internal LED |
|
Pulse-pair resolution: |
10 ns |
|
Flow cell: |
10 mm path, 25 µL volume with
flow-through cuvette |
|
Filters: |
Your waveband choice of 1 excitation
filter and 1 emission filter (call for options) |
|
Computer interface: |
RS-232 to Windows 98/Me/2000/XP/Vista OS |
|
